11Agent 13
When first introduced to Kate (a.k.a. Agent 13) in Captain America: The Winter Soldier she hangs up the phone and says to Steve she was on the phone to her aunt, who is kind of an insomniac. It is then revealed in Civil War that Kate is the niece of Peggy Carter.
12Queen Frigga
In Thor (2011), Queen Frigga is visibly uncomfortable when Odin states that Thor is his firstborn child. In Thor: Ragnarok (2017) we learn that Odin’s and Frigga’s firstborn was Hela.
13Ghost
In Ant-Man and The Wasp (2018) Ghost makes the motion of opening the double doors as she ghosts through them.
14Aunt May
In Spider-Man: Homecoming, Aunt May is reading ‘The Woman in the Dunes’ by Kobo Abe - which is about an entomologist who gets caught like an insect - at a time in the plot where Peter Parker is almost ‘caught’ as Spider-Man.
15Hela
Thor Ragnarök: Hela's bruises disappear once she reaches Asgard, along with the cracks on her skin. Previously it is mentioned that her power increases when she's on Asgard.
16Shuri
In Black Panther, Shuri asks which side of the road should she drive on due to her education in England as England drive left and most of the countries drive right.
17Pepper Potts
In The Avengers (2012), Pepper Potts is barefoot in stark tower whilst Tony Stark wears shoes. This was done to mitigate the height difference between Gwyneth Paltrow and Robert Downey Jr.
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18Valkyrie
In Thor: Ragnarok, the carcass that Valkyrie lands on is a bilgesnipe, a creature described by Thor in The Avengers.
19Gamora
After stabbing Thanos, Gamora's sword doesn't have any blood stain or blood drops on it indicating its a fake reality.
20Black Widow
Black Widow casually saves both Falcon's and Captain America's lives in Captain America: The Winter Soldier. When Bucky is shooting at these 3, he targets black widow first. She dives forward. She then pushes cap and Sam's heads out of the line of fire (watch the head cushions explode).
